PhD student Jonas Roland Knudsen recently visited Prof. Paul Verkade’s lab at University of Bristol in England for six months - an exchange visit that he received financial support for from Danish Diabetes Academy. He was there to use different correlative imaging techniques to study protein whereabouts in skeletal muscle.
MULTIPLE BENEFICIAL OUTCOMES
The research turned out to have multiple beneficial outcomes. "Thanks to the support from Danish Diabetes Academy, I returned with new techniques in the toolbox, plenty of consolidated memories and intriguing research data", Jonas Knudsen says.
DEVELOPMENT OF NEW TECHNIQUE TO STUDY PROTEIN WHEREABOUTS IN HUMAN SKELETAL MUSCLE
Before his stay, Jonas Knudsen conducted an animal experiment with mice transiently overexpressing the main glucose transporter in the skeletal muscle (GLUT4). He used the samples from this study in Bristol to develop a new technique to identify the GLUT4 whereabouts in the skeletal muscle. "Alongside, my colleagues in the Molecular Physiology Section at University of Copenhagen conducted a human study and provided me with human muscle tissue. I then performed a similar analysis in the human muscle tissue to pinpoint the GLUT4 transporters to different compartments in the skeletal muscle after exercise and insulin stimulation", Jonas Knudsen reports.
EXTREMELY REWARDING AND CHALLENGING
Paul Verkade’s team has roots from many different fields including physics, chemistry, and biology. "Working in an environment with highly specialized people from different backgrounds was extremely rewarding and also challenging. In addition, the social unity was strong. I really enjoyed being part of the team both at the professional and personal level. I am grateful that the Danish Diabetes Academy gave me the opportunity to experience this adventure", Jonas Knudsen concludes.